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Methodology
DNA Sequencing
Each exon (2-11) of the gene is sequenced completely along with the flanking intronic regions (an average of 20 bp in each direction) in order to evaluate the status of splice sites (donor and acceptor.) The results are compared to the wild type (most frequent normal) DNA sequence of the gene. If a mutation is found in the tumor, the proprietary database is checked to see if this mutation has been associated with cancer in published reports or should be added to the database
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